Puc19 vector pdfs

puc18 vector biology discussion


puc19 vector features

pUC19 is a common plasmid cloning vector in E.coli that is 2686 bp and has a high copy number. It carries a 54 bp multiple cloning site polylinker. pUC19 is a small, high-copy number E. coli plasmid cloning vector containing portions of pBR322 and M13mp19 (1). It con- tains the pMB1 origin of replication pUC19 is a small, high copy cloning vector for replication in E. coli. It has been constructed using the ampicillin resistance gene and the pMB1 origin of coli strain HB101 by a standard plasmid purification procedure. Usage: pUC18 is a commonly used plasmid cloning vector in E. coli. Concentration: 1 µg/µl. PUC18/19 Vector Map. Multiple cloning Sites of puc18: Hindlil. Pael Sdal al Multiple cloning Sites of puc19: Eco241 Acc6511 Eco88|| events warming. Pael.pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and Print/export. Create a book · Download as PDF · Printable version digested pUC19 and NdeI digested pUC19 vectors by T4 DNA ligase. of plasmid size on ligation efficiency, cloning of pUC19 fragments of sizes ~3686, 4686, pUC19 is a commonly used, high copy number cloning vector. This linearized version was generated by PCR, and contains the blunt ends shown in the MCS