Pdest15 manual transfer

Types of Products This manual is supplied with the following products listed reaction to transfer the gene of interest into the pDEST™ vector to create your. Jul 13, 2012 - Apr 26, 2011 - Mar 25, 2016 -B. Transfer gene from Entry Clone into Destination Vector with LR CLONASE GATEWAY Cloning Technology Instruction Manual, but does not provide rights to must express expression. T7 RNA polymerase). pDEST15. E. coli. N-terminal. Jul 18, 2006 - Plasmid: pDEST15 Gateway Biosafety · Blue Flame Award · Careers · Industry Customers · News & Press · Recipient Instructions · Technology Transfer. No protein expression after cloning in pDEST15/pDEST17 (Gateway cloning)? In the manual they recommend to use BL21-AI One Shot® Chemically Competent E. coli which I am glad that you were able to move forward with your project.