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Lorena Betancor, Fernando Lopez-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente et al. Pages 57-64. PDF. Although in 1950s the method of enzyme immobilization was still dominated by physical methods, i.e. non-specific physical adsorption of enzymes or proteins on. 2. Methods of immobilization. 1. Absorption. 2. Covalent linkage. 3. Enzyme entrapment. 4. Encapsulation of enzyme. 1. Adsorption. 34. 100. pH 7.0. 75. 100. Dec 17, 2019 - Guisan, Guadalupe Penzol, Pilar Armisen, Agatha Bastida, Rosa M. Blanco, Roberto Fernandez-Lafuente et al. Pages 261-275. PDF. Jun 27, 2014 -Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over Mar 20, 2013 - Based on the type of application, the method of immobilization and support material can be selected. The immobilized enzymes can be separated from the reaction. Based on the type of application, the method of immobilization and support material can be selected. The immobilized enzymes can be separated from the reaction. At the beginning, only immobilized single enzymes were used, after. 1970s more complex systems including two-enzyme reactions with cofactor regeneration and